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CRL-2899 ™. BCL2 Jurkat is a T lymphocyte cell line that was isolated in 2003 from the peripheral blood of a 14-year-old, male patient with leukemia, acute T cell. It was derived by transfecting the human Jurkat T cells with the pSFFV-neo mammalian expression vector containing the human BCL-2 ORF insert and a neomycin-resistant gene.

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Mix gently. Centrifuge at 1,200 rpm for 5 minutes to pellet cells. Discard media and resuspend pellet gently in 10 ml warm medium. Divide cells into two T-25 flasks containing 5 ml warm media. Place in incubator. After two days, remove the media and add fresh media. When cells are 6-8x105 cells/ml, split them 1:4 with fresh media.

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